Semen analysis evaluates a man's sperm and semen. It is done to discover cause for infertility and to confirm success of vasectomy.


Semen analysis is an initial step in investigating why a couple has been unable to conceive a child. Abnormalities of sperm and semen can cause male infertility. Semen is the thick yellow-white male ejaculate containing sperm. Sperm are the male sex cells that fertilize the female egg (ovum). They contain the genetic information that the male will pass on to a child. Vasectomy is an operation done to sterilize a man by stopping the release of sperm into semen. Success of vasectomy is confirmed by the absence of sperm in semen.


The semen analysis test is usually done manually, though computerized test systems are available. Many laboratories base their procedures on standards published by the World Health Organization (WHO). The volume of semen in the entire ejaculate is measured. The appearance, color, thickness, and pH is noted. A pH test looks at the range from a very acid solution to a very alkaline solution. Semen, like many other body fluids, has a standard pH range that would be considered optimal for fertilization of the egg to take place. The thick semen is then allowed to liquify; this usually takes 20-60 minutes. Drops of semen are placed on a microscope slide and examined under the microscope. Motility, or movement, of 100 sperm are observed and graded in categories, such as rapid progressive or immotile. The structure of sperm (sperm morphology) is assessed by carefully examining sperm for abnormalities in the size and shape in the head, tail, and neck regions. WHO standards define normal as a specimen with less than 30% abnormal forms. An alternative classification system (Kruger's) measures the dimensions of sperm parts. Normal specimens are allowed 14% or less abnormalities. Sperm are counted by placing semen in a special counting chamber. The sperm within the chamber are counted under a microscope. White blood cells are recorded; these may indicate a reproductive tract infection. Laboratories may test for other biochemicals such as fructose, zinc, and citric acid. These are believed to contribute to sperm health and fertility. Results of semen analysis for infertility must be confirmed by a second analysis seven days to three months after the first. Sperm counts may vary from day to day. Semen analysis to confirm success of vasectomy is concerned only with discovering if sperm are still present. Semen is collected six weeks after surgery. If sperm are seen, another specimen is collected 2 to 4 weeks later. The test is repeated until two consecutive specimens are free of sperm.